Size Exclusion Chromatography in LPLC Systems
Size exclusion chromatography (SEC), also called gel filtration, is another essential technique used in LPLC. SEC separates molecules based on size, making it invaluable for purifying proteins, polysaccharides, and other macromolecules. The stationary phase consists of porous beads that allow smaller molecules to enter the pores while larger molecules pass through more quickly.
Because SEC does not rely on interactions with the stationary phase, it is a highly gentle method that preserves sample integrity. This makes it an ideal step for buffer exchange and desalting, which are necessary when preparing biomolecules for enzymatic assays, crystallization, or storage.
LPLC-based SEC is particularly advantageous for handling large sample volumes. Although it offers lower resolution than high-pressure SEC, it provides the scalability needed for preparative applications. For researchers working on protein complexes or polymer distribution studies, LPLC SEC remains a dependable and efficient method.